Rapid method for determining efficacy of a sterilization...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/00 (2006.01) A61L 2/26 (2006.01) C12Q 1/22 (2006.01)

Patent

CA 2002902

A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.

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