Rapid method for separation of small molecules using reverse...

Details Rapid method for separation of small molecules using reverse... Rapid method for separation of small molecules using reverse...

G - Physics

01

N

G01N 30/36 (2006.01) G01N 30/34 (2006.01)

Patent

CA 2318143

The invention is a rapid method for the separation of small organic compounds using gradient reverse phase HPLC. The method of the invention achieves a run time of one minute or less and a resolution with a peak production of at least 1 peak/2 seconds. The method is also able to achieve the separation of a compound from a mixture of compounds in an elution sample having a volume of 2 milliliters or less.

La présente invention concerne une méthode rapide de séparation de petits composés organiques utilisant la chromatographie liquide haute performance (CLHP) en phase inversée de gradient. La durée d'exécution de cette méthode est d'une minute ou moins, sa résolution s'exprimant par une production crête d'au moins 1 crête/2 secondes. On peut par ailleurs utiliser cette méthode pour séparer un composé d'un mélange de composés dans un échantillon d'élution présentant un volume de 2 millilitres ou moins.

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