Rapid methods for identifying modifiers of cellular...

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G01N 33/50 (2006.01)

Patent

CA 2291116

The invention provides a single-well, microscale method of determining the specific apoptotic activity of a cell. The method consists of contacting a cell population of about 1 x 105 cells for a time period of between about 30 minutes and 4 hours with a sufficient volume of medium containing an apoptotic specific diagnostic reagent and a diagnostic accessory reagent so as to cover the cell population, and determining the activity of the apoptotic specific diagnostic reagent. The invention also provides a method of identifying a compound which induces apoptosis. The invention further provides a rapid method of identifying a compound which inhibits apoptosis.

La présente invention concerne une méthode à puits unique et à petite échelle permettant de déterminer l'activité apoptotique spécifique d'une cellule. Le procédé consiste à mettre en contact, pour une durée comprise entre 30 minutes et 4 heures environ, une population cellulaire d'environ 1x10?5¿ cellules avec un volume suffisant d'un milieu renfermant un réactif diagnostique spécifique de l'apoptose et un réactif diagnostique auxiliaire, de manière à recouvrir la population cellulaire, puis à déterminer l'activité dudit réactif diagnostique spécifique. L'invention concerne également une méthode rapide permettant d'identifier un composé qui inhibe l'apoptose.

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