Rapid preparation of stem cell matrices for use in tissue...

A - Human Necessities – 61 – L

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A61L 27/38 (2006.01) C12N 5/071 (2010.01) C12N 5/0775 (2010.01) A01N 63/00 (2006.01) C12N 5/00 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2438904

The present invention describes a rapid method for preparing stem cell and physiologically acceptable matrix compositions for use in tissue and organ repair. Compared with previous tissue engineering materials, the stem cell- matrix compositions of the prestn invention do not require long-term incubation or cultivation in vitro prior to use in in vivo applications. The stem cells can be from numerous sources and may behomogeneous, heterrogeneous, autologous, and/or allogeneic in the matrix material. The stem cell-matrix compositions as described provide point of service utility for the practitioner, wherin the stem cells and matrix can be combined not long before use, therby alleviating costly and lengthy manufacturing procedures. In addition, the stem cells offer unique structural properties to the matrix composition which improves outcome and healing after use. Use of stem cells obtained from muscle affords contractility to the matrix composition.

La présente invention concerne une méthode rapide de préparation de cellules souches et de compositions de matrice acceptables d'un point de vue physiologique, destinées à être utilisées dans la réparation de tissus et d'organes. Comparées aux matières de génie tissulaire déjà existantes, les compositions de matrice de cellules souches de la présente invention ne nécessitent pas d'incubation ou de culture de longue durée in vitro avant utilisation dans des applications in vivo. Lesdites cellules souches peuvent provenir de nombreuses sources et peuvent être homogènes, hétérogènes, autologues, et/ou allogéniques dans la matière de matrice. Lesdites compositions de matrice de cellules souches selon l'invention, permettent d'obtenir un point de service utilitaire pour le praticien, les cellules souches et le support pouvant être combinés peu avant l'utilisation, atténuant ainsi les longues et coûteuses procédures de fabrication. De plus, les cellules souches confèrent des propriétés structurales uniques à la composition de matrice, qui améliorent le résultat clinique et la guérison après utilisation. L'utilisation de cellules souches obtenues à partir du muscle assure une certaine contractilité à la composition de matrice.

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