B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
B01D 11/00 (2006.01) B01D 61/00 (2006.01) C12N 9/68 (2006.01)
Patent
CA 2492752
A method for rapid purification of a blood component from blood is described in which the blood plasma is first separated from the cellular blood elements by any conventional means, such as centrifugation. An affinity cartridge is then activated with a molecule, such as an amino acid, which binds with a blood component such as plasminogen. The separated blood plasma is then passed through the affinity cartridge such that the blood component is retained by the affinity cartridge. Thereafter, the blood component is eluted from the affinity cartridge by passing a buffer solution containing a releasing agent through the affinity cartridge. This releasing agent disengages the blood component from the affinity cartridge. The releasing agent is then separated from the eluted solution by passing the eluted solution through a device, such as an ion exchange, gel filter, or size exclusion device. The isolated plasminogen solution is then concentrated by a factor of from 2 to 10. The separated blood component, e.g. plasminogen, is then converted to plasmin by adding a known amount of an enzyme to the solution from which the releasing agent has been removed.
L'invention concerne un procédé rapide de purification d'un composant sanguin, consistant à séparer le plasma sanguin des éléments sanguins cellulaires par n'importe quelle technique classique, telle que la centrifugation, à activer une cartouche d'affinité avec une molécule, telle qu'un acide aminé, se liant à un composant sanguin tel que le plasminogène, à faire passer le plasma sanguin séparé à travers cette cartouche d'affinité de sorte que le composant sanguin soit retenu par celle-ci, à éluer le composant sanguin de la cartouche d'affinité en faisant passer à travers ladite cartouche une solution tampon contenant un agent de libération dégageant le composant sanguin de la cartouche d'affinité, puis à séparer cet agent de libération de la solution éluée en faisant passer cette dernière à travers un dispositif, tel qu'un dispositif d'échange d'ions, de filtration en milieu gélifié ou d'exclusion diffusion. La solution de plasminogène isolée est ensuite concentrée par un facteur compris entre 2 et 10. Le composant sanguin séparé, p. ex. le plasminogène, est ensuite converti en plasmine par addition d'une quantité connue d'une enzyme à la solution de laquelle l'agent de libération a été retiré.
Bell Craig J.
Dailey Wendelin A.
Hartzer Michael K.
Trese Michael T.
Williams George A.
Nuvue Technologies Inc.
Ridout & Maybee Llp
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