Rapid propagation of sv3t3 cells for production of mif and taf

Details Rapid propagation of sv3t3 cells for production of mif and taf Rapid propagation of sv3t3 cells for production of mif and taf

C - Chemistry, Metallurgy

12

N

195/46

C12N 5/00 (2006.01) C07K 14/515 (2006.01) C07K 14/52 (2006.01) C12N 1/10 (2006.01)

Patent

CA 1093995

Abstract of the Disclosure SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an incolum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30°-38°C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium,whereby said cells are proliferated in aggregated and elaborate HIF and TAF in significantly detectable amounts.

290067

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