Rapid separation of dirofilaria immitis immune complexes

Details Rapid separation of dirofilaria immitis immune complexes Rapid separation of dirofilaria immitis immune complexes

A - Human Necessities

61

K

167/129

A61K 39/00 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01)

Patent

CA 1256371

Abstract of the Disclosure A method for the rapid separation of Dirofilaria immitis immune complexes in a sample of blood or bodily fluid from an animal infected with Dirofilaria immitis preparatory to assaying for the presence of circulating parasite antigens of Dirofilaria immitis involves the steps of (a) lowering the pH of the sample to below 3.0 to effect the separation of circulating parasite antigens of Dirofilaria immitis from antibodies therefor in the sample; (b) heating the sample to a temperature within the range of approximately 56°C to 90°C for a sufficient period of time to denature the separated antibodies; and (c) increasing the pH of the resulting sample to within the range of approximately 7 to 8 to produce a sample which may be assayed for the presence of circulating parasite antigens of Dirofilaria immitis without interference from the separated antibodies.

500391

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