Rapid subcloning using site-specific recombination

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) C12N 15/90 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2336520

The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene or genes of interest into any vector that contains a sequence-specific recombinase target site located downstream of a regulatory element so that the gene of interest may be regulated.

Cette invention concerne des compositions, y compris des vecteurs, ainsi que des procédés qui permettent d'effectuer un sous-clonage rapide in vivo et in vitro de séquences d'acide nucléique. Cette invention concerne plus précisément des vecteurs qui sont utilisés afin de contenir un gène présentant un intérêt, et qui comprennent un site cible de recombinase spécifique à une séquence. Ces vecteurs permettent de transférer rapidement le ou les gènes présentant un intérêt dans tout vecteur qui comprend un site cible de recombinase spécifique à une séquence situé en aval d'un élément régulateur, ceci de manière à pouvoir réguler le gène présentant un intérêt.

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