Rapid triglyceride assay for use in pulp pitch control

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/61 (2006.01) C12Q 1/44 (2006.01) D21C 9/08 (2006.01) D21H 21/02 (2006.01) G01N 33/34 (2006.01) G01N 33/46 (2006.01)

Patent

CA 2424775

Enzymatic methods are provided for determining the surface triglycerides content in a sample of wood pulp. The methods preferably comprise reacting triglycerides which are present on the surface of the wood pulp fibers in the sample in the presence of a lipase to form glycerol and fatty acids, and then determining the difference between the amount of free glycerol present in the sample and the amount of glycerol formed from said triglycerides. The method is useful as a quick, portable, accurate, and low cost assay for assessing the amount of triglycerides present at various sample points in pulp and paper mills, which advantageously serves as a diagnostic tool for use in controlling the undesirable deposition of pitch during the papermaking process.

La présente invention concerne des procédés enzymatiques permettant de déterminer le contenu de triglycérides en surface dans un échantillon de pâte de bois. Ces procédés consistent, de préférence, à faire réagir des triglycérides présents à la surface des fibres de pâte de bois de l'échantillon en présence d'une lipase de façon à former du glycérol et des acides gras, puis à déterminer la différence entre la quantité de glycérol libre présente dans l'échantillon et la quantité de glycérol formée à partir de ces triglycérides. Ce procédé convient comme dosage rapide, portable, précis et bon marché permettant d'évaluer la quantité de triglycérides présente en divers points d'échantillonnage dans des usines de pâte et de papier, qui sert avantageusement d'outil de diagnostic destiné à contrôler le dépôt indésirable de poix durant le processus de fabrication du papier.

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