Rat model with the onset of prostate cancer

Details Rat model with the onset of prostate cancer Rat model with the onset of prostate cancer

A - Human Necessities

01

K

A01K 67/027 (2006.01) C07K 14/025 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2400966

To provide a rat model with the onset of prostate cancer in which the prostate cancer including an invasive cancer can be developed and can be bred for generations stably. A rat model with the onset of prostate cancer which can develop prostate cancer including invasive cancer and can be bred for generations stably is established by: ligating an SV40 large T antigen gene onto the downstream of rat probasin gene promoter; the resulting PBSVT transgene is introduced into a fertilized egg of a Sprague-Dawley rat; after the introduction, the fertilized egg is transplanted into a recipient rat; a transgenic rat obtained from the recipient rat is then mated with a wild- type Sprague-Dawley rat; the transgenic offspring rats thus obtained are similarly can be bred for generations thereafter; and transgenic rats developing prostate cancer are selected through histopathological observation of the prostate.

L'invention concerne des modèles murins présentant les premiers symptômes du cancer de la prostate chez lesquels on peut induire un cancer de la prostate, notamment un cancer infiltrant et que l'on peut sous-cultiver dans un état stable. A cet effet, on ligature le gène codant l'antigène T de SV40 à la partie aval du promoteur de la probasine du rat, et l'on transfère le transgène PBSVT ainsi obtenu dans un ovule fertilisé d'un rat Sprague-Dawley. Après le transfert, l'ovule fertilisé est transplanté chez un hôte. Ensuite, on fait s'accoupler le rat transgénique obtenu avec un rat Sprague-Dawley sauvage, les rats transgéniques ainsi obtenus étant sous-cultivés de façon similaire par la suite. Les rats transgénique présentant les premiers symptômes du cancer de la prostate sont sélectionnés sur la base de l'observation des tissus prostatiques pathologiques, ce qui permet d'établir des modèles murins présentant les premiers symptômes du cancer de la prostate, chez lesquels on peut induire un cancer de la prostate, notamment un cancer infiltrant, et que l'on peut sous-cultiver dans un état stable.

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