Rat ob receptors and nucleotides encoding them

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/16 (2006.01) C07K 14/72 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2246226

The rat ob receptor gene has been isolated and cloned. Two different alleles have been identified: the wild-type, and the fa-allele which differs from the wild-type by only one base pair. The base pair change, however, introduces an MspI restriction site into the DNA sequence, and also results in an amino acid change. Also part of the invention are the novel receptors, vectors containing the nucleic acid encoding the receptors, host cells transformed with this gene, and assays which use the gene or protein and identify new ligands.

Gène récepteur de l'obésité du rat (ob) isolé et cloné. Deux différents allèles ont été identifiés: le type sauvage et l'allèle fa, qui diffère du précédent seulement par une paire de base. Le changement d'une paire de base introduit toutefois un site de restriction MspI dans la séquence ADN et entraîne également une modification d'acide aminé. L'invention porte également sur de nouveaux récepteurs, sur des vecteurs renfermant l'acide nucléique codant ces récepteurs, sur des cellules hôtes transformées par ce gène et sur des dosages mettant en oeuvre ce gène ou cette protéine pour identifier de nouveaux ligands.

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