Rationally-designed meganucleases with recognition sequences...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/22 (2006.01) A61K 38/46 (2006.01) A61K 48/00 (2006.01) C12N 15/09 (2006.01) C12N 15/55 (2006.01) C12N 15/90 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2703079

Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

L'invention propose des méganucléases LAGLIDADG conçues rationnellement et des procédés de fabrication de telles méganucléases. De plus, des procédés sont proposés pour utiliser les méganucléases pour générer des cellules recombinantes et des organismes ayant la séquence d'ADN voulue insérée dans un nombre limité de lieux au sein du génome, ainsi que des procédés de thérapie génique, pour le traitement d'infections pathogènes et pour des applications in vitro dans les domaines du diagnostic et de la recherche.

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