Rationally designed polysaccharide lyases derived from...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/88 (2006.01) A61K 38/51 (2006.01) C12N 15/60 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2493509

The invention relates to rationally designed polysaccharide lyases and uses thereof. In particular, the invention relates to modified chondroitinase B. The modified chondroitinase B enzymes of the invention are useful for a variety of purposes, including cleaving and sequencing polysaccharides such as glycosaminoglycans (GAGs) as well as removing polysaccharides from a solution. The invention also includes methods of inhibiting anticoagulant activity, inhibiting angiogenesis, treating cancer, and inhibiting maternal malarial infection.

L'invention concerne des lyases de polysaccharide conçues de façon rationnelle ainsi que leurs utilisations. Elle concerne, en particulier, une chondroïtinase B modifiée. Les enzymes chondroïtinase B modifiées sont utiles dans un certain nombre d'applications comprenant le clivage et le séquençage de polysaccharides, notamment des glycosaminoglycanes (GAG), ainsi que l'élimination de polysaccharides d'une solution. L'invention concerne aussi des procédés d'inhibition de l'activité coagulante, d'inhibition de l'angiogénèse, de traitement du cancer, et d'inhibition de l'infection paludéenne maternelle.

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