Reaction accelerator-enchanced activity of cholesterol...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/60 (2006.01) C12N 9/04 (2006.01) C12Q 1/26 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2375894

A method of a pretreatment of a sample for quantitating cholesterol characterized by, before measuring cholesterol contained in specific lipoproteins, treating the sample containing lipoproteins with an enzyme, the substrate of which is free cholesterol, optionally together with a reaction accelerator; a method for quantitating cholesterol in specific lipoproteins by using the above method; and a kit for quantitating cholesterol in specific lipoproteins to be used in the above quantification method. By using this quantification method, cholesterol in a specific fraction can be conveniently, accurately and efficiently quantitated fundamentally without resort to polyanion, etc. Thus, this method is appropriately usable in various automatic analyzers.

Ce procédé de traitement préalable d'un échantillon, aux fins de quantification du cholestérol, est caractérisé en ce que, avant de mesurer le cholestérol contenu dans des lipoprotéines spécifiques, on traite l'échantillon contenant ces lipoprotéines à l'aide d'une enzyme dont le substrat est dépourvu de cholestérol, éventuellement avec un accélérateur de réaction. L'invention concerne également un procédé de quantification du cholestérol dans des lipoprotéines spécifiques, à l'aide du procédé ci-dessus, ainsi qu'une trousse de quantification du cholestérol dans des lipoprotéines spécifiques, utile dans le procédé de quantification ci-dessus. Grâce à ce procédé de quantification, il est possible de quantifier de manière pratique, précise et efficace le cholestérol dans une fraction spécifique, fondamentalement sans avoir recours à un polyanion, etc.. Ainsi, ce procédé est notamment conçu pour être utilisé dans divers dispositifs automatiques d'analyse.

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