Reaction device, reaction method and method of synthesizing...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/09 (2006.01) B08B 3/04 (2006.01) C12M 1/00 (2006.01)

Patent

CA 2712572

The present invention provides a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution and the like in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate, wherein the projecting barrier has at least one cutoff portion and an inner space capable of holding a droplet, and at least the face holding the droplets of the substrate surface has a contact angle to pure water of from 90 to 150 degrees. A reaction method using the above reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet defined by the above-described projecting barriers by means of a magnet located on the opposite surface of the substrate to the surface having the projecting barriers to thereby conduct reactions and washings.

Cette invention concerne un dispositif et un procédé permettant de mettre parallèlement en oevre de multiples opérations de réaction et opérations de lavage sans laver ou remplacer un instrument utilisé pour transférer une solution ou autre à chaque opération. Un gabarit pour multi-réactions selon l'invention comporte une pluralité de barrières saillantes agencées en ligne sur une surface d'un substrat, lesdites barrières saillantes possédant au moins une encoche et un espace intérieur pouvant contenir des gouttes liquides, la face au moins de la surface du substrat contenant les gouttes liquides ayant un angle de contact avec de l'eau pure de 90 à 150°. Dans le procédé de mise en réaction utilisant le gabarit pour multi-réactions décrit ci-dessus, une substance immobilisée sur des billes magnétiques est successivement transférée dans les gouttes d'une solution contenant un agent abaissant la tensioactivité contenues dans l'espace prévu pour contenir les gouttes liquides dans les barrières saillantes décrites ci-dessus au moyen d'un aimant situé sur la surface du substrat opposée à la surface comportant les barrières saillantes décrites ci-dessus pour mettre ainsi en oevre les réactions et les lavages.

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