Reaction monitoring

G - Physics – 01 – N

Patent

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Details

G01N 21/65 (2006.01) G01J 3/44 (2006.01)

Patent

CA 2354459

This invention relates to a method for monitoring a chemical reaction of pharmaceutical relevance, comprising the steps of: consecutively obtaining Raman spectrum samples of a solution of chemical constituents contained within a reaction vessel, by irradiating, with substantially monochromatic radiation, said solution and detecting the scattered radiation; processing said Raman spectrum samples by means of a multivariate data analysis (MVDA) for consecutively generating at least a first main component related to a latent variable indicative of the progress of the reaction wherein the multivariate data analysis is independent from calibration using reference measurements of objects having a known composition; and determining the extent of the chemical reaction on basis of said at least a first main component and optionally on basis of additionally one or more main components generated from one or more previously run reactions of the same kind.

L'invention porte sur un procédé de suivi d'une réaction chimique à caractère pharmaceutique comportant les étapes suivantes: (1) obtention consécutive d'échantillons des spectres de Raman d'une solution de constituant chimiques présente dans le bac de réaction en l'irradiant par un rayonnement quasi monochromatique puis en détectant le rayonnement diffusé; (2) traitement desdits échantillons par analyse MVDA (analyse de données à variables multiples), puis élaboration d'au moins une première composante liée à une variable latente indicatrice du progrès de la réaction, l'analyse MVDA étant indépendante de l'étalonnage si on utilise des mesures relatives à des objets de composition connue; et (3) détermination de l'étendue de la réaction chimique sur la base d'au moins ce premier composant principal et facultativement sur la base d'un ou plusieurs composants principaux obtenus à partir de réactions antérieures de même nature.

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