Reaction vessels

B - Operations – Transporting – 01 – L

Patent

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Details

B01L 7/00 (2006.01) B01J 19/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/48 (2006.01) G05D 23/19 (2006.01) H05B 3/14 (2006.01) H05B 3/20 (2006.01) C12M 1/00 (2006.01)

Patent

CA 2273840

A reaction vessel for holding reagents is described, which vessel comprises an electrically conducting polymer capable of emitting heat when an electric current is passed through it. Suitably the reaction vessel comprises a reagent container, such as a capillary tube, slide or chip, in close contact with the electrically conducting polymer. For example, the polymer may be in the form of a film which is wrapped around the tube to form a sheath. This provides a readily controllable heating supply which may be heated and cooled to desired temperatures rapidly. An apparatus suitable for thermal cycling reactions, such as the polymerase chain reaction (PCR) and comprising one or more reaction vessels as described above, as well as methods for carrying out such reactions are also described and claimed.

Cuve à réaction servant à contenir des réactifs et comprenant un polymère électriquement conducteur capable d'émettre de la chaleur quand il est traversé par un courant électrique. Cette cuve à réaction comprend un réservoir de réactif, tel qu'un tube capillaire, une plaquette ou une pastille, placé à proximité du polymère électriquement conducteur. Ce dernier peut, par exemple, se présenter sous la forme d'un feuil enroulé autour du tube, de manière à constituer une gaine. Ceci permet d'obtenir une alimentation en chaleur pouvant être commandée sans difficultés qu'on peut réchauffer et refroidir rapidement à la température souhaitée. Dispositif approprié à des réactions comportant des variations cycliques de température, telle que l'amplification en chaîne par polymérase (PCR) et comprenant une ou plusieurs de ces cuves à réaction, ainsi que procédés servant mettre en application ces réactions.

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