Reagent and method for the analytic determination of...

C - Chemistry – Metallurgy – 07 – D

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C07D 231/44 (2006.01) C12Q 1/28 (2006.01) C12Q 1/54 (2006.01) C12Q 1/60 (2006.01) C12Q 1/62 (2006.01)

Patent

CA 1125770

Abstract A 3-aminobenzoic acid or a 3-aminobenzenesulfonic acid, when reacted with a 4-aminoantipyrine in aqueous medium in the presence of hydrogen peroxide forms a novel colored dye which is believed to be a charge-transfer complex of free radicals formed from the 3-aminoaromatic acid and the 4- aminoantipyrine. The dye and the process by which it is produced are useful in analytical procedures for hydrogen peroxide, as well as in enzymatic analyses involving the formation of hydrogen peroxide.

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