Reagent and method of using same for the automatic flow...

Details Reagent and method of using same for the automatic flow... Reagent and method of using same for the automatic flow...

C - Chemistry, Metallurgy

12

Q

150/13, 150/3

C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2030381

The invention relates to a reagent and to a method of using same for automatically determining at least one leukocyte sub-population from a total blood sample. Thanks to this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.

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