Reagent and process for the determination of hemoglobin in...

Details Reagent and process for the determination of hemoglobin in... Reagent and process for the determination of hemoglobin in...

G - Physics

01

N

150/11

G01N 33/72 (2006.01)

Patent

CA 1319089

62-3653j Case 4444 REAGENT AND PROCESS FOR THE DETERMINATION OF HEMOGLOBIN IN BLOOD ABSTRACT OF THE DISCLOSURE A reagent system for determining the hemoglobin content of whole blood comprising an aqueous solution of polyvinylprrolidone at a pH sufficient to denature hemoglobin in whole blood. A method is also provided wherein a blood sample is treated with an aqueous solution of polyvinylprrolidone at a basic pH sufficient to denature the hemoglobin present in the whole blood to form a stable product whereafter the absorbance of the stable product of the blood sample and polyvinylprrolidone polymer reagent system is measured at a wavelength of approximately 575 nm to determine the hemoglobin contentration.

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