C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.5
C12Q 1/48 (2006.01)
Patent
CA 1215617
ABSTRACT Process and reagent for the determination of .gamma.-glutamyl transferase The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a coloured compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity. The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents: .gamma.-GT-arylamide 1 - 100 mmol/l. .gamma.-glutamyl acceptor (glycylglycine) 10 - 500 mmol/l. coupling component 1 - 500 mmol/l. oxidation agent 0.1 - 500 mmol/l. buffer 10 - 1000 mmol/l.
436783
Deneke Ulfert
Nagel Rolf
Rittersdorf Walter
Boehringer Mannheim G.m.b.h.
Marcoux & Sher Swabey Mitchell Houle
LandOfFree
Reagent and process for the determination of the activity of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagent and process for the determination of the activity of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent and process for the determination of the activity of... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1328781