Reagent and process for the quantitative colorimetric...

C - Chemistry – Metallurgy – 12 – Q

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150/15, 150/3.2

C12Q 1/50 (2006.01) C12N 9/96 (2006.01)

Patent

CA 1120388

ABSTRACT OF THE DISCLOSURE An all-in-one reagent is provided for the quantitative chlorometric determination of creatine phosphokinase in blood serum or plasma wherein the tetrazolium dye, 2-(p-iodophonyl)-3-(p-nitrophonyl)-5-phonyl tetrazolium chloride (INT), preferably together with an electron transport such as diapho- rase, and compatible substances which are enzymatically converted by creatine phosphokinase to an end product that will reduce said dye from its normally colorless state to a colored reduced state are combined into a homogenous composition which can be dried to a stable reagent.

358539

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