Reagent for assaying antigen and method of assaying antigen

Details Reagent for assaying antigen and method of assaying antigen Reagent for assaying antigen and method of assaying antigen

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2591872

It is intended to provide a reagent and an assay method whereby an antigen can be conveniently and accurately assayed without resorting to any special antibody. Using two or more carriers having different particle sizes, a carrier having a smaller particle size is sensitized with at least one monoclonal antibody, which is selected from among three monoclonal antibodies being reactive with both of a free antigen and a complex antigen and having different recognition sites from each other, while a carrier having a larger particle size is sensitized with the remainder monoclonal antibodies, thereby providing an assay reagent and an assay method whereby the reactivities of a free antigen and a complex antigen can be controlled.

L'invention propose un réactif et un procédé de dosage qui permettent de doser de manière précise et pratique un antigène sans avoir recours à un anticorps spécial quelconque. Deux vecteurs ou davantage ayant des particules de tailles différentes sont utilisés ; un vecteur ayant des particules de plus petite taille est sensibilisé à au moins un anticorps monoclonal, ce dernier étant choisi parmi trois anticorps monoclonaux qui réagissent à la fois avec un antigène libre et un antigène complexe et qui ont des sites de reconnaissance différents les uns des autres, tandis qu'un vecteur ayant des particules de plus grande taille est sensibilisé aux anticorps monoclonaux restants, ce qui fournit un réactif de dosage et un procédé de dosage grâce auxquels la réactivité d'un antigène libre et celle d'un antigène complexe peuvent être contrôlées.

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