Reagent for assaying creatine kinase

C - Chemistry – Metallurgy – 12 – Q

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150/3.2

C12Q 1/50 (2006.01) C12N 9/12 (2006.01) C12Q 1/32 (2006.01) C12Q 1/54 (2006.01)

Patent

CA 1252700

ABSTRACT OF THE DISCLOSURE A reagent system for assaying creatine kinase is disclosed. The reagent system consisting essentially of a first reagent comprising glucose-6-phosphate dehydrogenase, .beta.-nicotinamideadenine dinucleotide (phosphate), and adeno- sine diphosphate, and a second reagent comprising creatine phosphate, said second reagent being maintained at a pH of from 7.5 to 10, and at least one of said first reagent and said second reagent containing glucokinase and glucose. The creatine kinase-assaying reagent exhibits remarkably im- proved stability in a dissolved state so that it can be prepared tin large quantities and can be conventionally utilized to cope with urgent clinical examinations.

491777

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