Reagent for measuring immature leukocytes

Details Reagent for measuring immature leukocytes Reagent for measuring immature leukocytes

C - Chemistry, Metallurgy

12

Q

C12Q 1/06 (2006.01) C12N 5/00 (2006.01) G01N 1/28 (2006.01) G01N 15/10 (2006.01) G01N 33/49 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2119390

A reagent for measuring immature leukocytes which com- prises: (1) a polyoxyethylene-based nonionic surfactant having the general formula (I) in a sufficient amount capable of fixing cytoplasms and cell membranes of immature leukocytes: R1-R2-(CH2CH2O)n-H (I) where R1 is an alkyl, alkenyl or alkynyl group having 10 to 25 carbon atoms; R2 is -O-, -(C6H4)-O- or -COO-; and n is an integer of 10 to 40, (2) a solubilizing agent in a suffi- cient amount capable of damaging cell membranes of blood cells other than immature leukocytes and shrinking them, (3) an amino acid in a sufficient amount capable of stabilizing cytoplasm and cell membrane of immature leukocytes, and (4) an aqueous medium, which adjusts pH value in the range of 5.0 to 9.0, osmolarity in the range of 150 to 600 mOsm/kg and electric conductivity in the range of 6.0 to 9.0 mS/cm, respectively.

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