Reagent for the determination of lipase and process for the...

C - Chemistry – Metallurgy – 12 – Q

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150/3.2, 150/15.

C12Q 1/34 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 1139201

ABSTRACT The present invention provides a recanstitutable dry reagent especially for the turbidimetric determin- ation of lipase, which reagent forms an emulsion upon adding water and comprises substrate oil, protective colloid, emulsifier and activator, said reagent contain- ing: 0.2 to 10°%o by weight of liquid triglyceride, 20 to 90% by weight of protective colloid, 5 to 60% by weight of an alkali metal salt of a bile acid, 0.001 to 0.1% by weight of colipase, 0.1 to 2.0%. by weight of preserving agent, 5 to 2.0% by weight of urea, 3 to 50% by weight of buffer substance for pH 5.0 to 10.5 and 0.5 to 5% by weight of activator. The present invention also provides processes for the preparation of this reagent.

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