Reagent for the isolation of rna

C - Chemistry – Metallurgy – 07 – H

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C07H 21/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2435241

The present invention provides RNA extraction reagents, methods and kits that are especially useful for extracting RNA. The reagents, methods and kits of the present invention are especially useful for extracting RNA, for example, cytoplasmic RNA, from difficult materials, from plants, especially, difficult plant tissues, such as those containing phenolics, tannins, polysaccharides (such as starch) and resins. Comparative high yields are obtainable according to the present invention when compared to conventional reagents and methods. The RNA preparations obtained in accordance with the present invention are also of high quality as demonstrated by superior A260/280 results and by gel electrophoresis.

La présente invention se rapporte à des réactifs d'extraction d'ARN ainsi qu'à des procédés et à des trousses qui s'avèrent particulièrement utiles pour l'extraction d'ARN. Ces réactifs, procédés et trousses sont tout particulièrement utiles pour l'extraction d'ARN, par exemple de l'ARN cytoplasmique, à partir de matières difficiles, à partir de plantes, particulièrement de tissus végétaux difficiles, tels que ceux contenant des dérivés phénoliques, des tannins, des polysaccharides (tels que l'amidon) et des résines. Conformément à la présente invention, il est possible d'obtenir des rendements comparativement élevés par rapport aux réactifs et procédés classiques. Les préparations d'ARN obtenues conformément à la présente invention sont également de qualité élevée ainsi que le démontrent les résultats supérieurs de calcul du rapport A¿260/280? ainsi que l'électrophorèse sur gel.

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