Reagent mixtures for glucose assay

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/54 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2110908

ABSTRACT OF THE DISCLOSURE The present invention relates to a test reagent mixture composition comprising the enzymes glucose oxidase and peroxidase and a chromogen which interacts with the hydrogen peroxide from the oxidation of the blood glucose by the glucose oxidase, characterised in that the glucose oxidase and the peroxidase are present in proportions which provide from 300 to 700 International Units (IUs) of glucose oxidase and at least 20 International Units of peroxidase and in that the chromogen is present in an amount which provides from 12 to 20 grams of active chromogen per 500,000 International Units of glucose oxidase. Preferably, the composition is put up in a low molecular weight gelatin matrix and is impregnated into a micro-porous carrier membrane.

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