Reagent system and method for identification, enumeration...

Details Reagent system and method for identification, enumeration... Reagent system and method for identification, enumeration...

G - Physics

01

N

167/39

G01N 33/554 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1310904

ABSTRACT Specified classes and subclasses of leukocyte blood cells are identified by immunohematology procedures, based on utilization of antigenic determinants on the cell surface, their reactivity with antibodies which fluoresce under known circumstances, and identified by utilization of principles of flow cytometry or morphology. This invention particularly concerns improvements in the lysing and fixing method used prior to detection and identifying of the cells. In this method, a whole blood sample first is incubated with a reagent including antibodies to the cell subclass to be identified, the antibodies having been directly or indirectly made fluorescently responsive to a particular light (e.g. argon ion laser). The red blood cells then are lysed with a reagent containing saponin. Next follows a leukocyte fixing treatment, preferably using a cross-linking dialdehyde composition, such as glyoxal or glutaraldehyde. Details are given as to time, temperature, concentration, and the use of additives and stains, as well as the use of particular monoclonal antibodies in the preferred procedure.

482842

Affiliated with

Also associated with

Rating

Reagent system and method for identification, enumeration... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reagent system and method for identification, enumeration..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent system and method for identification, enumeration... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1255482