Reagent system and method for the differentiation and...

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G01N 33/49 (2006.01) G01N 1/30 (2006.01) G01N 1/38 (2006.01) G01N 15/00 (2006.01) G01N 15/06 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2237473

Whole blood is mixed with a reticulocyte reagent system that has a reticulocyte staining reagent and a diluent reagent, used in combination. This mixture is incubated at room temperature for between about 15 minutes to about 4 hours. The incubated mixture is then analyzed and the light scattering properties of the cells are detected, collected, differentiated and quantitized. Data gathering includes, at least, 10 and 90 degree light scatter detection.

On mélange du sang total avec un système de réactif concernant des réticulocytes, qui comprend un réactif de coloration de réticulocytes et un réactif de dilution utilisés en combinaison. On fait incuber ce mélange à température ambiante entre 15 minutes et 4 heures environ. On analyse ensuite le mélange incubé et on détecte des propriétés des cellules concernant la diffusion de la lumière, ou les collecte, différencie et quantifie. L'acquisition des données porte au moins sur la détection de la diffusion lumineuse à 10 et 90 degrés.

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