Reagent system and process for adenosine triphosphate...

Details Reagent system and process for adenosine triphosphate... Reagent system and process for adenosine triphosphate...

C - Chemistry, Metallurgy

12

Q

C12Q 1/66 (2006.01) C12N 9/96 (2006.01) C12Q 1/06 (2006.01) G01N 21/76 (2006.01)

Patent

CA 2483570

A reagent system comprises a first reagent which includes a high pH phosphate buffer, and a second reagent which includes luciferase, luciferin, a magnesium salt and an enzyme stabilizer. The second reagent has a low pH and a buffer with a pK which is near the optimum pH for activity of luciferase. The reagent system may be used in a process for measuring total adenosine triphosphate (ATP) and/or dissolved extracellular ATP, in a fluid containing microorganisms. The reagent system may also be used in a microbiological remediation or production process.

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