Reagents

Details Reagents Reagents

C - Chemistry, Metallurgy

07

K

195/35, 167/44,

C07K 17/00 (2006.01) B01D 15/08 (2006.01) B01J 20/32 (2006.01) C07K 1/22 (2006.01) C07K 16/12 (2006.01) C12N 9/36 (2006.01) G01N 33/531 (2006.01) G01N 33/543 (2006.01) G01N 33/552 (2006.01)

Patent

CA 2032389

ABSTRACT Improved affinity purification media are provided by the use of small specific binding agents, especially Fv antibody fragments or single domain antibody fragments, immobilised on porous carriers having pore sizes in the range 30-1000 angstroms, preferably 30-300 angstroms. Silica is a preferred carrier. The small fragments are able to penetrate the pores and maximise the effective surface area of the carrier, and the microporous silica is sufficiently robust to be used at high pressure, so enabling the speed and/or throughput of a purification procedure to be increased.

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