Reagents and method for detecting polychlornated biphenyls

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/435 (2006.01) C07C 59/68 (2006.01) C07D 311/82 (2006.01) C07K 16/00 (2006.01) C07K 16/44 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2041783

ABSTRACT OF THE DISCLOSURE Reagents and an immunoassay for detecting the presence or amount of polychlorinated biphenyls in a test sample. The assay is performed by adding a known concentration of a tracer labeled with a detectable moiety and a known concentration of an analyte-specific antibody to a test sample to form a mixture, incubating the mixture to form labeled tracer-antibody and analyte-antibody complexes, and determining the presence or amount of tracer-antibody complexes formed as a measure of the presence or amount of analyte in the test sample. Reagents provided include tracers, immunogens and an additive compound useful in preventing non-specific binding ofthe polychlorinated biphenyls to proteins which may be present in the test sample. A kit for performing the assay also is provided. -61-

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