Reagents and methods for identifying and modulating...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2410480

This invention identifies growth-inhibitory genes induced by retinoids. The invention provides reagents and methods for identifying compounds other than retinoids that induce expression of these cellular genes. The invention also provides reagents that are recombinant mammalian cells containing recombinant expression constructs that express a reporter gene under the transcriptional control of a promoter for a gene that is regulated by retinoids, and methods for using such cells to identify compounds other than retinoids that modulate expression of these cellular genes.

L'invention concerne des gènes inhibiteurs de croissance induits par les rétinoïdes. L'invention concerne des réactifs et des méthodes permettant d'identifier des composés autres que les rétinoïdes induisant l'expression de ces gènes cellulaires. L'invention concerne également des réactifs composés de cellules recombinées de mammifères contenant des gènes d'expression hybrides recombinés exprimant un gène rapporteur sous régulation transcriptionnelle d'un promoteur pour un gène régulé par les rétinoïdes. L'invention concerne également des méthodes d'utilisation de ces cellules pour identifier des composés autres que les rétinoïdes modulant l'expression de ces gènes cellulaires.

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