Reagents and methods for improving reproducibility and...

Details Reagents and methods for improving reproducibility and... Reagents and methods for improving reproducibility and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2584576

An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.

L'invention porte sur un additif qui permet d'empêcher les erreurs d'amorçage lors d'amplifications par réaction en chaîne de la polymérase ("polymerase chain reaction" ou PCR) et sur des dosages renfermant un oligonucléotide en épingle à cheveux comportant un tronc à double hélice supérieur à six nucléotides de longueur et possédant une extrémité de tronc stabilisée. L'additif de l'invention améliore les amplifications PCR, y compris les amplifications LATE-PCR, lorsqu'il est ajouté aux mélanges de réaction d'amplification initiaux. L'additif précité peut être inclus dans des jeux d'oligonucléotides et des trousses d'amplification et de dosage PCR.

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