G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/558 (2006.01) G01N 33/538 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01)
Patent
CA 2109924
2109924 9221769 PCTABS00017 The present invention includes novel assays and reagents using a capture reagent, involving a specific binding member attached to a polymeric anionic substance, and a solid phase material containing a capture or reaction zone including a polymeric cationic substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. With an appropriate indicator reagent, sandwich, competitive and indirect assays can be performed. Preferably, the indicator reagent includes a nonspecific binding blocker to reduce the nonspecific binding between the indicator reagent and the solid phase, without interfering with the reaction between the capture reagent and the solid phase. Suitable nonspecific binding blockers include unbound or free polyanionic materials such as dextran sulfate, heparin, carboxymethyl dextran, carboxymethyl cellulose, pentosan polysulfate, inositol hexasulfate or .beta.-cyclodextrin sulfate.
Adamczyk Janina
Berry Daniel S.
Fico Rosario
Jou Yi-Her
Stroupe Stephen Denham
Abbott Laboratories
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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