Reagents for specific binding assays

Details Reagents for specific binding assays Reagents for specific binding assays

G - Physics

01

N

G01N 33/543 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2151197

A sensitive assay method has been discovered that reduces the amount of non-specific binding present in an assay, the method comprising detecting an analyte present in a sample through a specific binding reaction in which either an analogue of the analyte or a specific binding partner of the analyte is immobilized on a solid-phase and said specific binding reaction produces a detectable product immobilized on said solid-phase that may be correlated to the amount of analyte present in the sample wherein said assay employs an effective amount of a surfactant selected from the group consisting of a polyoxyethylene-alkylether, a polyalkylene oxide-modified polydimethylsiloxane block copolymer, a polyalkylene oxide-modified polymethylsiloxane block copolymer, and mixtures thereof to reduce non-specific binding.

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