Reagents for use in binding assays to diphenylhydantoin

C - Chemistry – Metallurgy – 07 – H

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C07H 17/06 (2006.01) A61K 47/48 (2006.01) C07D 233/72 (2006.01) C07H 17/075 (2006.01) C07H 21/00 (2006.01) G01N 33/531 (2006.01) G01N 33/533 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1121810

ABSTRACT OF THE DISCLOSURE Reagents for use in binding assays, particularly immuno- assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including .beta.-galactosyl-umbelliferone- diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N1, N3 and o-phenyl derivatives of diphenylhydantoin for preparing same. N1 and N3-.omega.- aminoalkyl and o-(.omega.-aminoalkoxy)- phenyl derivatives are prepared and coupled by amide linkage to a .beta.-galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N1 and N3-.omega.-carboxyalkyl and o-(.omega.-carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic poly- amino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.

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