Real-time detection of nucleic acids and proteins

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2541969

The present invention provides a method for real-time detection of an independent target nucleic acid or target nucleic acid linked to a secondary structure through signal amplification (direct detection) or through detection of the target nucleic acid sequence which has been the subject of an amplification process. A probe including a detectable marker is hybridized to either an independent target nucleic acid or a linked target nucleic acid to provide verification of the presence of the target nucleic acid and/or secondary structure to which the target nucleic acid is linked within either isothermal or non-isothermal environments of homogeneous or heterogeneous systems.

La présente invention concerne une méthode de détection en temps réel d'un acide nucléique cible indépendant ou d'un acide nucléique cible lié à une structure secondaire par amplification du signal (détection directe) ou par détection de la séquence d'acide nucléique cible qui a fait l'objet d'un processus d'amplification. Une sonde comprenant un marqueur détectable est hybridée avec un acide nucléique cible indépendant ou avec un acide nucléique cible lié afin de vérifier la présence de l'acide nucléique cible et/ou de la structure secondaire à laquelle l'acide nucléique cible est lié dans des environnements isothermes ou non isothermes de systèmes homogènes ou hétérogènes.

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