Real time electronic cell sensing systems and applications...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/34 (2006.01) C12M 1/42 (2006.01)

Patent

CA 2550274

The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays (102) on a nonconducting substrate (101), in which each of the arrays (102) has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices (416) comprising multiple wells each having an electrode array (102), an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state.

L'invention concerne des dispositifs, des systèmes et des procédés de dosage de cellules au moyen du contrôle de l'impédance de substrats cellulaires. Dans un aspect, l'invention concerne des dispositifs de contrôle d'impédance de substrats cellulaires qui comprennent des réseaux d'électrodes placés sur un substrat non-conducteur, chacun des réseaux présentant une résistance d'électrode presque uniforme dans l'ensemble du réseau. Dans un autre aspect, l'invention porte sur des systèmes de contrôle de substrats cellulaires comprenant un ou plusieurs dispositifs de contrôle de substrats cellulaires qui comportent plusieurs puits dotés chacun d'un réseau d'électrodes, un analyseur d'impédance, un dispositif qui relie les réseaux de puits individuels à l'analyseur d'impédance, et un logiciel pour contrôler le dispositif et l'analyseur d'impédance. Dans un autre aspect, l'invention concerne des dosages cellulaires qui utilisent le contrôle d'impédance pour détecter des changements du comportement ou de l'état cellulaire.

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