Real-time hpv pcr assays

Details Real-time hpv pcr assays Real-time hpv pcr assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2604761

The present invention relates a fluorescent multiplex PCR assay for detecting the presence of an HPV type in a sample using multiple fluorophores to simultaneously detect a plurality of HPV genes of the same HPV type, wherein the HPV type is selected from the group consisting of: HPV33, HPV35, HPV39, HPV51, HPV56, and HPV59. The present invention also relates to oligonucleotide primers and probes specific to said HPV types for use in the methods of the present invention.

L'invention concerne un dosage PCR multiplex fluorescent permettant de détecter la présence d'un type de papillomavirus humain (HPV) dans un échantillon au moyen de fluorophores multiples, afin de détecter simultanément une pluralité de gènes du HPV du même type de HPV, le type de HPV étant choisi dans le groupe constitué par: HPV33, HPV35, HPV39, HPV51, HPV56 et HPV59. L'invention concerne également des amorces et des sondes oligonucléotidiques spécifiques desdits types de HPV, destinées à être utilisées dans les procédés selon l'invention.

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