Real time nucleic acid detection in vivo using protein...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2627410

The present invention relates to a method to detect nucleic acid molecules, such as RNA molecules in vivo using real time protein complementation methods. The invention further relates to methods for detecting nucleic acids, for example RNA in real-time in living cells with a high sensitivity, using a novel split biomolecular conjugate of the invention.

L'invention concerne un procédé permettant de détecter des molécules d'acides nucléiques, telles que des molécules d'ARN in vivo au moyen de procédés de complémentation protéique en temps réel. Cette invention concerne également des procédé permettant de détecter des acides nucléiques, par exemple un ARN en temps réel dans des cellules vivantes avec une grande sensibilité, au moyen d'un nouveau conjugué biomoléculaire clivé décrit dans cette invention.

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