C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/70 (2006.01)
Patent
CA 2569747
Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1- 89, and 96~104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein.
L'invention se rapporte à des compositions comprenant des amorces et des sondes qui peuvent interagir avec des acides nucléiques, par exemple les acides nucléiques qui codent la transcriptase inverse ou la protéase du VIH. Cette invention concerne également un oligonucléotide comprenant une quelconque séquence nucléotidique parmi SEQ ID NO:1-89 et 96-104. La présente invention se rapporte aussi aux oligonucléotides comprenant les nucléotides parmi SEQ ID NO:1-89 et 96-104. Chacun desdits oligonuclétides est une sonde ou une amorce. Cette invention se rapporte également à des mélanges d'amorces et de sondes, destinés à être utilisés dans des réactions RT-PCR et des réactions PCR primaires. L'invention concerne en outre des procédés pour détecter de manière spécifique plusieurs mutations du VIH. Les mutations au sein de la transcriptase inverse et de la protéase du VIH peuvent être détectées au moyen desdits procédés.
Heneine Walid
Johnson Jeffrey A.
Ridout & Maybee Llp
The Government Of The United States As Represented By The Secret Centers For Disease Control
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