Real time pcr with the addition of pyrophosphatase

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C12Q 1/42 (2006.01)

Patent

CA 2515242

The present invention is directed to a method and a kit for amplifying and detecting a target nucleic acid, wherein the composition containing reagents to perform and monitor nucleic acid amplification in real time comprises at least a first hybridization probe labeled with a first fluorescent entity and a Pyrophosphatase.

La présente invention concerne une méthode et une trousse pour l'amplification et la détection d'un acide nucléique ciblé, où la composition qui contient des réactifs permettant d'effectuer et de surveiller l'amplification en temps réel comprend au moins une première sonde d'hybridation marquée à l'aide d'une première entité fluorescente et d'une pyrophosphatase.

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