Real time polymerase chain reaction detection of legionella...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2747651

Materials and processes are provided for the detection of Legionella species in a sample. The inventive process includes exposing a sample to a forward primer and a reverse primer to yield an amplicon. The amplicon is detectable by at least one probe. The inventive process detects multiple species of Legionella bacteria or is specific for Legionella pneumophila. A kit is provided with inventive primers and probes for the detection of a Legionella species in a sample. Image

L'invention porte sur des matières et des procédés pour la détection d'espèces Legionella dans un échantillon. Le présent procédé comprend l'exposition d'un échantillon à une amorce sens et à une amorce antisens pour produire un amplicon. L'amplicon est détectable par au moins une sonde. Le procédé de l'invention détecte de multiples espèces de bactéries Legionella ou est spécifique pour Legionella pneumophila. L'invention porte également sur un kit renfermant des amorces et des sondes de l'invention pour la détection d'une espèce Legionella dans un échantillon.

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