Real-time sequencing methods and systems

Details Real-time sequencing methods and systems Real-time sequencing methods and systems

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2760155

The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance or inhibit such reactions. In certain preferred embodiments, RNA templates are used in single-molecule real-time sequencing reactions.

La présente invention porte d'une manière générale sur des compositions, des procédés et des systèmes pour réaliser une analyse en temps réel à molécule unique d'une pluralité de réactions biologiques différentes. La capacité à analyser de telles réactions fournit une opportunité d'étudier ces réactions ainsi que d'identifier potentiellement des facteurs et/ou des approches pour avoir une répercussion sur de telles réactions, par exemple, pour soit activer soit inhiber de telles réactions. Dans certains modes de réalisation préférés, des matrices d'ARN sont utilisées dans des réactions de séquençage en temps réel à molécule unique.

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