Receptor-based interaction trap

Details Receptor-based interaction trap Receptor-based interaction trap

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C07K 14/705 (2006.01) C07K 14/71 (2006.01) C07K 14/715 (2006.01) C07K 19/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2407872

The present invention relates to a recombinant receptor, comprising an extracellular ligand-binding domain and a cytoplasmic domain that comprises a heterologous bait polypeptide, which receptor is activated by binding of a ligand to said ligand binding domain and by binding of a prey polypeptide to said heterologous bait peptide. The present invention also relates to a method to detect compound-compound binding using said recombinant receptor.

La présente invention concerne un récepteur de recombinaison, présentant un domaine de liaison de ligand extracellulaire et un domaine cytoplasmique qui comprend un polypeptide appât hétérologue. Ce récepteur est activé par liaison d'un ligand audit domaine de liaison de ligand et par liaison d'un polypeptide proie audit peptide appât hétérologue. La présente invention concerne également un procédé de détection de liaison composé-composé, qui met en oeuvre ledit récepteur de recombinaison.

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