Receptor on the surface of activated t-cells: acts-4

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 39/395 (2006.01) C07K 14/705 (2006.01) C07K 14/725 (2006.01) C07K 16/28 (2006.01) C07K 16/46 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 5/20 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2175577

The invention provides purified ACT-4 receptor polypeptides, antibodies against these polypeptides and nucleic acids encoding ACT-4 receptor polypeptides. Also provided are methods of diagnosis and treatment using the same. ACT-4 receptors are preferentially expressed on the surface of activated CD4+ T-cells. ACT-4 receptors are usually expressed at low levels on the surface of activated CD8+ cells, and are usually substantially absent on resting T-cells, and on monocytes and B-cells (resting or activated). An exemplary ACT-4 receptor, termed ACT-4-h-1, has a signal sequence, an extracellular domain comprising three disulfide-bonded intrachain loops, a transmembrane domain, and an intracellular domain.

L'invention se rapporte à des polypeptides récepteurs purifiés, ACT-4, à des anticorps dirigés contre ces polypeptides, ainsi qu'à des acides nucléiques codant ces polypeptides récepteurs. L'invention se rapporte également à des procédés diagnostiques et thérapeutiques comprenant leur utilisation. Les récepteurs ACT-4 sont de préférence exprimés sur la surface de lymphocytes T CD4?+¿ activés. Ils sont généralement exprimés en faibles quantités sur la surface de lymphocytes T CD8?+¿ activés, et sont généralement pratiquement absents sur des lymphocytes T au repos ainsi que sur des monocytes et des lymphocytes B (au repos ou activés). Un récepteur ACT-4 modèle, appelé ACT-4-h-1, comprend une séquence signal, un domaine extracellulaire comprenant trois boucles intrachaîne à liaison bisulfure, un domaine transmembranaire ainsi qu'un domaine intracellulaire.

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