Recipient block and method for preparation thereof

Details Recipient block and method for preparation thereof Recipient block and method for preparation thereof

G - Physics

01

N

G01N 1/00 (2006.01)

Patent

CA 2529892

Disclosed is a recipient block for arraying a certain tissue sample into a desired position on a tissue microarray slide, comprising(a) an additive and (b) a wax. The present invention also discloses a method of preparing a recipient block, including (1) preparing an aqueous solution of an 5 additive; (2) pouring the aqueous solution of the additive into a mold and cooling the mold to gelate the aqueous solution of the additive; (3) dehydrating the resulting additive gel in alcohol; (4) immersing the dehydrated additive gel in an organic solvent to make the dehydrated additive gel transparent; (5) penetrating a wax into the transparent additive gel to provide a block; and (6) providing a plurality of cylindrical holes in the block. Further, the present invention discloses a method of preparing a tissue microarray block, including (1) arraying each of certain tissue samples into each of the cylindrical holes of the recipient block; and (2) heating the recipient block in which the tissue samples have been arrayed at 50~C to 70~C for 20 to 40 min and cooling the recipient block for embedding of the tissue samples.

L'invention concerne un bloc receveur permettant de placer un certain échantillon de tissu dans une position voulue sur une lame à microréseau de tissu, et qui comprend (a) un additif et (b) une cire. L'invention concerne aussi un procédé de préparation d'un tel bloc, qui comporte les étapes consistant à : (1) préparer une solution aqueuse d'un additif ; (2) verser la solution aqueuse d'additif dans un moule et refroidir le moule pour gélifier la solution aqueuse d'additif ; (3) déshydrater le gel d'additif obtenu dans de l'alcool ; (4) immerger le gel d'additif déshydraté dans un solvant organique pour rendre ledit gel transparent ; (5) faire pénétrer une cire dans le gel d'additif transparent afin de former un bloc ; et (6) former une pluralité de trous cylindriques dans le bloc. De plus, l'invention concerne un procédé de préparation d'un bloc à microréseau de tissu, qui comporte les étapes consistant à : (1) mettre en place chacun des échantillons de tissu dans chacun des trous cylindriques du bloc ; (2) chauffer le bloc contenant les échantillons de tissu à une température comprise entre 50 ·C et 70 ·C pendant 20 à 40 min, et refroidir le bloc afin d'incorporer les échantillons de tissu.

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