Recognition sequences for i-crei-derived meganucleases and...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/22 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2730921

Methods of cleaving double-stranded DNA that can be recognized and cleaved by a rationally-designed, I-Crel-de-rived meganuclease are provided. Also provided are recombinant nucleic acids, cells, and organisms containing such recombinant nucleic acids, as well as cells and organisms produced using such meganucleases. Also provided are methods of conducting a cus-tom-designed, I-Crel-derived meganuclease business.

L'invention concerne des méthodes de clivage d'ADN double brin qui peut être reconnu et clivé par une méganucléase dérivée d'I-Crel, conçue rationnellement. L'invention concerne également des acides nucléiques recombinants, des cellules et des organismes contenant lesdits acides nucléiques recombinants, ainsi que des cellules et des organismes produits à l'aide desdites méganucléases. L'invention concerne en outre des méthodes de mise en oeuvre d'un commerce de méganucléases dérivées d'I-Crel, personnalisées.

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